Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells regulate the Th17/Treg cell balance partly through hepatocyte growth factor in vitro

doi: 10.1186/s13287-020-01612-y

Figure Lengend Snippet: The modulatory effect of HGF secreted by MSCs on Th17/Treg cell differentiation in CD4+ T cells analysed by flow cytometry. The results showed that the percentage of CD4+CD25+Foxp3+ cells was significantly increased in the CD4+ T cell population, but the MSC-induced effect was significantly inhibited by an anti-HGF antibody ( p < 0.05, a , c ). The percentage of CD4+CD3+RORrt+ cells was significantly decreased after MSC coculture. However, the MSC-induced effect was significantly inhibited by the anti-HGF antibody ( p < 0.05, b , d ) ( n = 3; * p < 0.05 or ** p < 0.01 vs. the CD4+ group; # p < 0.05 vs. the MSC+CD4+ group). MSCs, mesenchymal stem cells; rmHGF, recombinant mouse hepatocyte growth factor

Article Snippet: LPS (100 ng/ml) and 100 pg/ml recombinant mouse HGF (rmHGF; Sino Biological, China) or the polyclonal rabbit anti-HGF antibody (ab83760, 100 ng/ml) were added to the cocultures of stimulated CD4+ T cells.

Techniques: Cell Differentiation, Flow Cytometry, Recombinant

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells regulate the Th17/Treg cell balance partly through hepatocyte growth factor in vitro

doi: 10.1186/s13287-020-01612-y

Figure Lengend Snippet: The regulatory effect of HGF secreted by MSCs on cytokine mRNA expression in CD4+ T cells analysed by qPCR. The results showed that MSCs significantly increased IL-10 ( p < 0.05, a ) and TGF-β ( p < 0.01, b ) mRNA expression and inhibited IL-17 ( p < 0.01, c ) and IL-6 ( p < 0.05, d ) mRNA expression in CD4+ T cells. However, the MSC-induced effects were inhibited by an anti-HGF antibody ( p < 0.05 or p < 0.01) ( n = 3; * p < 0.05 or ** p < 0.01 vs. the CD4+ group; # p < 0.05 or ## p < 0.01 vs. the MSC+CD4+ group). MSCs, mesenchymal stem cells; rmHGF, recombinant mouse hepatocyte growth factor; qPCR, quantitative polymerase chain reaction

Article Snippet: LPS (100 ng/ml) and 100 pg/ml recombinant mouse HGF (rmHGF; Sino Biological, China) or the polyclonal rabbit anti-HGF antibody (ab83760, 100 ng/ml) were added to the cocultures of stimulated CD4+ T cells.

Techniques: Expressing, Recombinant, Real-time Polymerase Chain Reaction

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells regulate the Th17/Treg cell balance partly through hepatocyte growth factor in vitro

doi: 10.1186/s13287-020-01612-y

Figure Lengend Snippet: The regulatory effect of HGF secreted by MSCs on cytokine production in CD4+ T cells detected by ELISA. We found that MSCs significantly increased the CD4+ T cell secretion of IL-10 ( p < 0.01, a ) and TGF-β ( p < 0.01, b ) but inhibited the CD4+ T cell secretion of IL-17 ( p < 0.05, c ) and IL-6 ( p < 0.01, d ). However, the MSC-induced effects were inhibited by an anti-HGF antibody ( p < 0.05 or p < 0.01) ( n = 3; * p < 0.05 or ** p < 0.01 vs. the CD4+ group; # p < 0.05 or ## p < 0.01 vs. the MSC+CD4+ group). MSCs, mesenchymal stem cells; rmHGF, recombinant mouse hepatocyte growth factor

Article Snippet: LPS (100 ng/ml) and 100 pg/ml recombinant mouse HGF (rmHGF; Sino Biological, China) or the polyclonal rabbit anti-HGF antibody (ab83760, 100 ng/ml) were added to the cocultures of stimulated CD4+ T cells.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells regulate the Th17/Treg cell balance partly through hepatocyte growth factor in vitro

doi: 10.1186/s13287-020-01612-y

Figure Lengend Snippet: The regulatory effect of HGF secreted by MSCs on neutrophil activities in cells cocultured with CD4+ T cells and analysed with flow cytometry. The results indicated that CD4+ T cells cocultured with MSCs significantly inhibited neutrophil phagocytic ( p < 0.05, a ) and oxidative burst activities ( p < 0.01, b ). However, the effects of the CD4+ T cells were inhibited by an anti-HGF antibody ( p < 0.05 or p < 0.01) ( n = 3; * p < 0.05 or ** p < 0.01 vs. the CD4+ group; # p < 0.05 or ## p < 0.01 vs. the MSC+CD4+ group). MSCs, mesenchymal stem cells; rmHGF, recombinant mouse hepatocyte growth factor

Article Snippet: LPS (100 ng/ml) and 100 pg/ml recombinant mouse HGF (rmHGF; Sino Biological, China) or the polyclonal rabbit anti-HGF antibody (ab83760, 100 ng/ml) were added to the cocultures of stimulated CD4+ T cells.

Techniques: Flow Cytometry, Recombinant

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells regulate the Th17/Treg cell balance partly through hepatocyte growth factor in vitro

doi: 10.1186/s13287-020-01612-y

Figure Lengend Snippet: The regulatory effect of HGF secreted by MSCs on CD40+ and CD80+ monocyte activation in cocultures with CD4+ T cells and analysed with flow cytometry. The results showed that after being cocultured with MSCs, CD4+ T cells significantly inhibited monocyte expression of CD40 ( p < 0.05, a ) and CD80 ( p < 0.05, b ). However, the CD4+ T cell-mediated effect was inhibited by an anti-HGF antibody ( p < 0.01) ( n = 3; * p < 0.05 vs. the CD4+ group; ## p < 0.01 vs. the MSC+CD4+ group). MSCs, mesenchymal stem cells; rmHGF, recombinant mouse hepatocyte growth factor

Article Snippet: LPS (100 ng/ml) and 100 pg/ml recombinant mouse HGF (rmHGF; Sino Biological, China) or the polyclonal rabbit anti-HGF antibody (ab83760, 100 ng/ml) were added to the cocultures of stimulated CD4+ T cells.

Techniques: Activation Assay, Flow Cytometry, Expressing, Recombinant

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells regulate the Th17/Treg cell balance partly through hepatocyte growth factor in vitro

doi: 10.1186/s13287-020-01612-y

Figure Lengend Snippet: The regulatory effect of HGF secreted by MSCs on MHC-II and TLR-2 expression in monocytes cocultured with CD4+ T cells and analysed with flow cytometry. The results showed that after being cocultured with MSCs, CD4+ T cells significantly inhibited monocyte expression of MHC-II ( p < 0.01, a ) and TLR2 ( p < 0.05, b ). However, the CD4+ T cell-mediated effect was inhibited by an anti-HGF antibody ( p < 0.05 or p < 0.01) ( n = 3; * p < 0.05 or ** p < 0.01 vs. the CD4+ group; ## p < 0.01 vs. the MSC+CD4+ group). MSCs, mesenchymal stem cells; rmHGF, recombinant mouse hepatocyte growth factor

Article Snippet: LPS (100 ng/ml) and 100 pg/ml recombinant mouse HGF (rmHGF; Sino Biological, China) or the polyclonal rabbit anti-HGF antibody (ab83760, 100 ng/ml) were added to the cocultures of stimulated CD4+ T cells.

Techniques: Expressing, Flow Cytometry, Recombinant

Journal: International Journal of Molecular Sciences

Article Title: Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia

doi: 10.3390/ijms23158093

Figure Lengend Snippet: HGF, CCL2 and TGF-α measurements by ELISA. ( A ) Boxplot comparing HGF levels in BALF of MCAO vs. sham mice (N = 49) ( B ) Dotplot representing HGF measurement by Olink ® panel ( X -axis) and measurement by ELISA ( Y axis) (N = 53). ( C ) Boxplot comparing CCL2 levels in lung of MCAO vs. sham mice (N = 30) ( D ) Dot plot representing CCL2 measurement by Olink ® panel ( X axis) and ELISA measurement ( Y axis) (N = 30). ( E ) Boxplot comparing TGF-α levels in lung of MCAO vs. sham mice (N = 30) ( F ) Dot plot representing TGF-α measurement by the Olink ® panel ( X axis) and ELISA measurement ( Y axis) (N = 30). The boxplots showed increased HGF, a trend towards increased CCL2 and decreased TGF-a values in MCAO vs. sham animals, while the scatterplots showed positive correlations for HGF and CCL2 and a negative correlation for TGF-a. HGF: Hepatocyte Growth Factor, CCL2: C-C Motif Chemokine Ligand 2, TGF-α: Transforming Growth Factor α, ELISA: Enzyme-Linked ImmunoSorbent Assay, BALF: bronchoalveolar lavage fluid, NPX: normalized protein expression, MCAO: middle cerebral artery occlusion.

Article Snippet: Three proteins were evaluated per sample using ELISA: HGF (Mouse HGF DuoSet ELISA, R&D Systems, TGF- α (Mouse TGF-α ELISA Kit, Elabscience, Bethesda, MD, USA), CCL2 (Mouse CCL2/JE/MCP-1 DuoSet ELISA, R&D Systems, Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: The Tohoku journal of experimental medicine

Article Title: Osteopontin as a key mediator for vasculogenic mimicry in hepatocellular carcinoma.

doi: 10.1620/tjem.224.29

Figure Lengend Snippet: Fig. 7. Immunohistochemical staining for VM in HCC and normal liver tissues. (A) VM expression was identified in a HCC lesion by laminin immunostain (red arrow). (B) The same HCC lesion in (A) showing VM expression by laminin im- munostain did not express the endothelial cell marker CD31. Hepatic sinusoids were CD34 positive (yellow arrow). (C) The same HCC lesion in (A) was positive for hepatocyte. (D) A HCC lesion without VM expression by laminin im- munostain. Bars = 50 μm.

Article Snippet: After washing in 0.1 M phosphate-buffered saline (PBS), pH 7.4, and exposure to 3% normal goat serum for 20 min to reduce non-specific binding, the slides were incubated for 2 h at 37°C with a 1:100 dilution of anti-laminin monoclonal antibody (Millipore), or a 1:100 dilution of anti-CD34 antibody (Santa Cruz Biotechnology, Santa Cruz, CA), or a working solution of anti-hepatocyte antibody (Zhongshan Goldenbridge Biotechnology CO Ltd, ready to use), or a 1:300 dilution of anti-OPN antibody (Santa Cruz), or a 1:300 dilution of anti-MMP-2 antibody (Santa Cruz), or a 1:300 dilution of anti-MMP-9 antibody (Santa Cruz), or a 1:300 dilution of anti-uPA antibody (Santa Cruz).

Techniques: Immunohistochemical staining, Staining, Expressing, Marker